S.Ramalingam

Education

Degree	Year	University	Field of Study
Ph D	2005	Anna University, Chennai	Biotechnology
M. Tech.	1994	Anna University, Chennai	Biotechnology
B.E (Chem)	1991	Annamalai University, Chidambaram	Chemical Technology

Position and Employment

Institution Place	Position	From (Date)	To (date)
Anna University, Chennai	Associate Professor	Dec 2012	Till Date
Anna University, Chennai	Assistant Professor	Sep 2009	Nov 2012
Anna University, Chennai	Lecturer Senior Grade	Nov 2008	Nov 2009
Anna University, Chennai	Lecturer	Nov 2003	Oct 2008
Alembic Chemical Works Ltd.,Vadodara, Gujarat	Biochemical Engineer (Pilot Plant)	Oct 1997	Mar 1999
Synbiotics Ltd., Vadodara, Gujarat	Assistant, Manager- Fermentation	Jan 1997	Sep 1997
J.K.PHARMACHEM Ltd., Cuddalore, Tamil Nadu	Production officer - Fermentation	May 1994	Dec 1996

Professional Experience

The investigator is a chemical engineer turned biotechnologist specialized in fermentation of genetically manipulated systems in bacteria especially metabolic engineering. The investigator has a well structured industrial career spanning five years in three giant pharmaceutical companies. Earlier, he has worked in fermentation and purification of recombinant proteins in E. coli. Currently work is focussed on metabolic engineering for synthesis of green chemicals.

He is offering bioprocess engineering and metabolic engineering course for B.Tech and M.Tech , M.S and PhD students at Anna University. Further he is also involved in the Marie Curie International Research staff exchange program of the ‘Food Research Exchange Programme’ between India and Europe offered by the European Union for metabolic engineering work package. This project involves collaboration in teaching and research.

Research Interest

Currently his research group is focusing on heterologous and synthetic pathway engineering by kinetic modeling and multiple gene assembly in hosts E. coli and Lactic acid bacteria. The group has pioneered engineering of acrylate pathway and the major thrust is in overcoming the bottlenecks associated with acrylate pathway leading to an array green chemicals such as propionic acid, beta alainine and other products. Further, work is also focused on developing redox balanced microbial chassis for acrylate pathway in Lactobacillus reuteri by utilizing glycerol as carbon source in milk whey medium. Products include green chemicals such as1,3-Propanediol, 3 Hydroxypropionic acid, 2-oxobutanoic acid, alpha amino butyric acid in E. coli /Lactococcus lactis. The focus is also on biopolymers, poly-3 Hydroxypropionic acid and poly hydroxyalkanoic acid. An exclusive fungal fermentation facility has been created for process optimization and currently work is in progress fro the development of technology for the production of cellulases in Trichoderma viride

Funded Projects as Principal Investigator

S.No	Title of Project	Funding Agency	Amount(in lakhs)	Year of sanction	Duration
1.	Understanding Optimal Recombinant Protein Production in E. coli by Metabolic Flux Analysis: A Systems Approach	DBT	12.98	JAN 2009	3 Years
2.	Increasing yield of Reuterin (3-Hydroxypropionaldehyde) in metabolically Engineered Lactobacillus reuteri.	UGC	7.64	JAN 2009	3 Years
3.	Production of 1, 3-Propanediol in Metabolically Engineered Lactobacillus reuteri using GlycerolFeedstock (Jointly between AU &IITM )	DST	37.48	OCT 2009	3 Years
4.	Metabolic Engineering of Lactococcus lactis for the Production Propionic Acid (Jointly between AU &IITM )	DBT	66 .0	SEP 2013	3 years
5.	DBT Virtual Enzyme Centre- Development of Enzyme Formulations for treatment ofLigno-cellulosic Biomass (Multi institutional Project)	DBT	62.61	OCT 2014	3 years
6.	Food Research Exchange Programme Between and (METABOLIC ENGINEERING)	EUROPEAN CBT AU & LMC(CAFS) &FDRS DTU Systems Biology	NA	2010 to2014	3 years

Invited Talks

Delivered invited lecture titled “Metabolic engineering of Lactobacillus reuteri for the enhanced production of 1,3-Propanediol” on 23rd January 2014 at The National Food Institute, Technical University of Denmark, Lyngby, Denmark.

Delivered invited lecture titled “Development of fermentation Processes in Genetically Engineered Lactic Acid Bacteria for Food Applications” in the 5th ASIAHORCs joint symposium held on 26-28 November 2013, Nusa Dua, Bali. Indonesia

Delivered invited lecture titled “Metabolic Engineering Strategies for the Production of Ethanol ” in February 2013 at Department of Food Technology, Wageningen University, Wageningen, Netherlands

Foreign trips by Faculty and their students between 2012 – 2014.

Currently on a visit to Wageningen University, Netherlands, under EASTWESTFOOD staff exchange programme funded by European commission from 4th January- 30th January.

Participated in the ASIAHORC Symposium on “Food Sciences” held at Bali, Indonesia during November 26-28, 2013.

Visited Waganingen University, Netherlands, during 10th February -31st March 2013 Along with 3 Phd students under EASTWESTFOOD programme funded by European commission. During this visit we had attended course work offered by Waganingen University and actively involved in discussion with various scientists for establishing joint proposals and collaborations.

Visited Wageningen University ,Netherlands,during 4th January - 31st January 2014

Publications in International and National Journals in the Last Five Years:

Ramalingam.S., Gautam. P., Mukherjee. K. J. and Jayaraman.G (2007), Effects of Post-Induction Feed Strategies on Secretory Production of Recombinant Streptokinase in Escherichia coli, Biochemical Engineering Journal 33(1), 34-41.
Ramalingam. S., Vikram. M, Vignesh Babu and Sivasankari (2007), Flux balance analysis for maximizing polyhydroxyalkonates production in Pseudomonas putida, Indian Journal of Biotechnology, 10, 70-74.
Thangadurai. C., Suthakaran P, Pankaj Barfal, Anandaraj B Ramalingam S and V. Murugan (2008), Rare codon priority and specific position in 5’ of the target gene modulates the protein expression in E. coli. Biophysical Biochemical Research Communication 376(4), 647-652.
Bhuvanesh.S, Arunkumar.C., Kaliraj.P, Ramalingam .S (2010), Production and single-step purification of Brugia malayi abundant larval transcript (ALT-2) using hydrophobic interaction chromatography, Journal of Industrial Microbiology and Biotechnology 37(10), 1053-1059.
Hema.V, Vijayalakshmi.K, Gopi.GR, Ramachandran.KB, Jayaraman.G and Ramalingam.S (2011), Glycerol conversion to 1,3-Propanediol is enhanced by the expression of heterologous alcohol dehydrogenase in Lactobacillus reuteri, AMB Express 1(1), 37.
Geethalakshmi. S, Narendran. S, Pappa. N and Ramalingam.S (2011), Development of hybrid neural network model to predict feeding method in fed-batch cultivation for enhanced recombinant streptokinase productivity in Escherichia coli, Journal of Chemical Technology and Biotechnology 87(2), 280-285.
Vijayalakshmi.K, Hema.V,Djurdjevic.I, Jayamani.E, K.B.Ramachandran , Buckel.W, Jayaraman.G and Ramalingam.S (2013) “Engineering E. coli with acrylate pathway genes for propionic acid synthesis and impact on mixed acid fermentation”. Applied Microbial Biotechnology, 97,3, 1191-1200 .
Jolly.J,Hitzmann.B, Ramalingam.S and K.B.Ramachandran,(2013) ”Biosynthsis of 1,3-propanediol from glycerol with Lactobacillus reuteri: Effect of Operating Variables. Journal of Bioscience and Bioengineering,(Accepted for Publication Ms. Ref. No.: JBIOSC-D-13-00679R1)
Vaidyanathan, Hema, Suppuram, P, Gopinarayanan, V.E., Purushothaman, Y and Ramalingam.S (2013), “Enhancement of 1,3 –propanediol production in Lactobacillus reuteri by fed batch cultivation”, Asian Journal of Microbiology, Biotechnology and Environmental Sciences, vol.15, no.4, pp. 57-62.
Kannan.A, Ramalingam.S and Gautam. P (2014), “A study on Pseudomonas aeruginosa biofilm formation using atomic force microscopy.” Asian Journal of Microbiology, Biotechnology & Environmental Sciences, Vol.16, pp 379..
Kannan.A, Karumanchi S.L., Krishna.V., Thiruvengadam .K, Ramalingam.S and Pennathur Gautam.P (2014)“.Nanoscale investigation on Pseudomonas aeruginosa biofilm formed on porous silicon using Atomic Force Microscopy.” Scanning, Vol.36, pp 551.
Gopi.G.R., Ganesh.N., Pandiaraj.S., Sowmya.B., Brajesh. R.G. and Ramalingam.S (2015) A Study on Enhanced Expression of 3-Hydroxypropionic Acid Pathway genes and impact on its production in Lactobacillus reutri. Journal of Food Technology and Biotechnology, Vol. 53(3)
Gopi.G.R., Ganesh.N., Pandiaraj.S., Sowmya.B., Brajesh. R.G. and Ramalingam.S (2015) Bio-transformation of Glycerol to 3-Hydroxypropionic Acid Using Resting Cells of Lactobacillus reuteri. Current Microbiology.,Vol.71(4):517-23.
Eiteman.M.A and Ramalingam.S (2015) “Microbial Production of lactic acid.”Biotechnology letters 37:955–972
Kannan. A, Karumanchi. S.L., Ramalingam. S., Gautam. P., (2016) “Quantitative study on the effect of calcium and magnesium palmitate on the formation of Pseudomonas aeruginosa biofilm”. J Microbiol Immunol Infect. 49(6):988-991.
P. Gururaj.P.,Ramalingam.S., Ganesan Nandhini Devi.G. , Gautam.P.,(2016) ‘’Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp.’’ Brazilian Journal of Microbiology,Vol. 47, (3), Jul–Sep. 2016, Pp 647–657

Conferences

Delivered talk for invited lecture titled “Design and construction of heterologous acrylate pathway in E. coli and L. lactis for propionic acid synthesis” at Mepco Schlenk Engineering College Sivakasi to deliver a guest lecture on 17th March 2017. We will be > > honored to have you here for the event entitled ‘*National seminar on > > Contemporary issues in Bioprocesses Engineering -NCBE’17
Delivered invited lecture titled “ Kinetic modeling of heterologous acrylate pathway in E. coli and lactic acid bacteria to elucidate the bottlenecks limiting the flux in the pathway. “ Bioprocessing India held at Mohali on 15-17 December,2016
Delivered Invited talk- titled “Synthesis Of Biofuels By Multistep Pathway Engineering” on 21.7.2017 at International Conference on Renewable Energy and Sustainable Environment RESE 2017, Dr Mahalingam College of Engineering and Technology, International Conference, Pollachi, 20-22nd July 2017
Conducted one day symposium titled SYNTHETIC BIOLOGY AND PATHWAY ENGINEERING on 2.12.2016.