• Immunotechnology (vaccine/diagnostics development)
• Immunopharmacology
• Structure Biology
• Genetic Engineering
• Recombinant protein production
• Papers in International Journals: 14
• Papers in conferences -International:8
• Papers in conferences -National:7
• Patents applied:2
• Guided Dissertations : M.Tech – 10 and B.Tech - 10
PDB Structure database:
• PDB ID – 4FYU : N.Yousef, Prince R. Prabhu, A.Ahmed, S.Iqbal, P.Kaliraj, C.Betzel : “Crystal structure of Thioredoxin from Wuchereria bancrofti at 2.0 A” RCSB 073523, 2012
• PDB ID – 3OHQ : Prince R. P, M.Sakthidevi, J.Madhumathi, M.Perbandt, C.Betzel, P.Kalira: “Structure of wuchereria bancrofti pi-class glutathione s-transferase” RCSB061130, 2010
Genbank Nucleotide database:
•GU596489 : Madhumathi J., Prince R. P, Yamuna K.M, Mahesh P. and Kaliraj P., Synthetic construct ALT epitope protein 1 gene , complete cds. “Mapping B epitopes and evaluation of recombinant epitope construct of Bm ALT-2 in experimental filariasis”, 2010.
• EU877233 : . Prince R. P, Jeya Prita P. J, Madhumathi J, and Kaliraj P.,Brugia malayi clone CBT_Shp1a (BmShp1a) mRNA, complete cds “Immunological studies of recombinant Brugia malayi Sheath Protein”, 2008
•HM590635 : Sakthidevi,M., Jeyanthi,N., Prabhu, P. R. and Kaliraj, P. Wuchereria bancrofti glutathione-S-transferase pi class (GST) gene, GST-2a allele, complete cds, “Genetic polymorphic studies of Wuchereria bancrofti parasite genes”, 2010.
•HM590636 : Sakthidevi,M., Jeyanthi,N., Prabhu,P. R. and Kaliraj, P. Wuchereria bancrofti glutathione-S-transferase pi class (GST) gene, GST-2b allele, complete cds, “Genetic polymorphic studies of Wuchereria bancrofti parasite genes”, 2010.
• HM590638 : Sakthidevi,M., Jeyanthi,N., Prabhu, P. R. and Kaliraj, P. Wuchereria bancrofti glutathione-S-transferase pi class (GST) gene, GST-2c allele, complete cds, “Genetic polymorphic studies of Wuchereria bancrofti parasite genes”, 2010
IEDB Epitope database:
• Reference ID: 1020928, Epitope ID: 136943; Madhumathi J, Prince PR, Anugraha G, Kiran P, Rao DN, Reddy MVR, Kaliraj P
• Reference ID: 1021236, Epitope ID: 138844; Madhumathi J, Prince PR, Nageswara Rao D, Kaliraj P
•Molecular Biology & Genetic engineering: Plasmid and Genomic DNA Isolation, Restriction analysis of plasmid and genomic DNA, PCR, Primer designing, DNA Electrophoresis, PCR, Cloning in eukaryotic and prokaryotic expression vectors (pRSET, pVAX vectors), RTPCR, cDNA preparation and evaluation, TOPO cloning
•Structural biology and crystallography: DLS (dynamic light scattering ); crystallization studies – screening and optimization of crystallization conditions, Hanging drop vapour diffusion methods, Crystal screening by preliminary X-ray diffraction studies – bench top rotating anode at cryo and room temperature, X-ray diffraction data collection using synchrotron radiation at beam line, Processing of diffraction data (Mosflm), Structure elucidation (CCP4i suite, Pymol)
•Bioprocess Technology: Optimisation of conditions for growth and large-scale production of DNA vaccine and recombinant proteins. Recombinant protein expression in different E.coli hosts (GJ1158, BL21DE3, BL21Plys, TG2, TOP10), Pichia pastoris (X33). Protein Purification: FPLC, Ion-exchange chromatography, IMAC, SDS-PAGE, Native-PAGE
•Immunology: PBMC, Spleenocytes proliferation, Preparation of Peritoneal exudates, Cytokine estimation(ELISA), Raising antibodies & purification, Western/Dot blotting, ELISA
•Microbiology and cell biology: Isolation and identification of Bacteria, Maintenance of bacterial cultures and Optimization of medium conditions, Maintenance of cell lines, Transfection of mammalian cells (CHO cell line), PBMC Isolation, proliferation and toxicity studies (MTT reduction assay)
•Animal Handling: Trained to handle Gerbils and BALB/c mice, Rabbit, for filarial protection studies (Micropore chamber preparation and implantation and parasite challenge studies), Rat and mice for inflammatory studies.
•Bioinformatics: DNA/ protein Sequence analysis (gene tool, clone manager), Multiple sequence alignment, BLAST tools, in-silico epitope prediction (DNA star)
• Indian Patent application No: 1367/CHE/2011 “Peptide immunogens of lymphatic filarial ALT-2 and lymphatic filarial vaccine composition comprising”- Madhumathi.J, Prince.R.Prabhu, Anugraha.G, Kaliraj.P. (Ref.No:E-12/102/2011-CHE).
• Indian Patent Apllication No: 1366/CHE/2011 “Dominant T epitope of filarial transglutaminase and chimeric peptide vaccines for lymphatic filariasis”- Madhumathi.J, Prince.R.Prabhu, Kaliraj.P. (Ref.No:E-12/103/2011-CHE).
Visiting Faculty
Department of Biotechnology
Anna University, Chennai 600025
princeprp@gmail.com
9444 852463